机读格式显示(MARC)
- 000 02129nam 2200289 a 4500
- 008 020625r20022001cc a b 001 0 eng d
- 020 __ |a 7506247690 (set)
- 099 __ |a CAL 022002142561
- 100 1_ |a Sambrook, Joseph.
- 245 10 |a Molecular cloning : |b a laboratory manual = 分子克隆 : 实验室手册 / |c [Joseph Sambrook, David W. Russell].
- 246 31 |a 分子克隆 : |b 实验室手册
- 260 __ |a 西安 : |b 世界图书出版公司, |c 2002.
- 300 __ |a 3 v. : |b ill. ; |c 28 cm.
- 504 __ |a Includes bibliographical references and index.
- 505 0_ |a Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors -- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Ch. 6. Preparation and analysis of eukaryotic genomic DNA -- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells -- Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction -- Ch. 9. Preparation of radiolabeled DNA and RNA probes -- Ch. 10. Working with synthetic oligonucleotide probes -- Ch. 11. Preparation of cDNA libraries and gene identification -- Ch. 12. DNA sequencing -- Ch. 13. Mutagenesis -- Ch. 14. Screening expression libraries -- Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli -- Ch. 16. Introducing cloned genes into cultured mammalian cells -- Ch. 17. Analysis of gene expression in cultured mammalian cells -- Ch. 18. Protein interaction technologies.
- 534 __ |p Reprint. Originally published: |c Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, c2001. |b 3rd ed. |z 0879695765 (cloth) |z 0879695773 (pbk)
- 650 _0 |a Molecular cloning |v Laboratory manuals.
- 650 12 |a Cloning, Molecular |v Laboratory Manuals.
- 700 1_ |a Russell, David W. |q (David William), |d 1957-
- 950 __ |a JHUL |b Q785 |c S187=3