MARC状态：审校 文献类型：西文图书 浏览次数：29
- 题名/责任者:
- Molecular cloning : a laboratory manual = 分子克隆 : 实验室手册 / [Joseph Sambrook, David W. Russell].
- 版本说明:
- 3rd ed.
- 出版发行项:
- 西安 : 世界图书出版公司, 2002.
- ISBN:
- 7506247690 (set)
- 载体形态项:
- 3 v. : ill. ; 28 cm.
- 变异题名:
- 分子克隆 : 实验室手册
- 个人责任者:
- Sambrook, Joseph.
- 附加个人名称:
- Russell, David W. (David William), 1957-
- 论题主题:
- Molecular cloning-Laboratory manuals.
- 论题主题:
- Cloning, Molecular-Laboratory Manuals.
- 中图法分类号:
- Q785
- 书目附注:
- Includes bibliographical references and index.
- 内容附注:
- Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors -- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Ch. 6. Preparation and analysis of eukaryotic genomic DNA -- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells -- Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction -- Ch. 9. Preparation of radiolabeled DNA and RNA probes -- Ch. 10. Working with synthetic oligonucleotide probes -- Ch. 11. Preparation of cDNA libraries and gene identification -- Ch. 12. DNA sequencing -- Ch. 13. Mutagenesis -- Ch. 14. Screening expression libraries -- Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli -- Ch. 16. Introducing cloned genes into cultured mammalian cells -- Ch. 17. Analysis of gene expression in cultured mammalian cells -- Ch. 18. Protein interaction technologies.
- 原版附注:
- Reprint. Originally published: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, c2001. 3rd ed. 0879695765 (cloth) 0879695773 (pbk)
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